Microscope, Digital, 3-D
Monday, September 11, 2006
A Digital 3D Microscope has been designed and developed with a confocal microscope system capable of rendering three-dimensional (3D) images. The images are obtained using a microscope equipped with oil-immersion ultraviolet (UV) optical system and UV illumination. A short-arc Xenon flash minimizes sample cell photo bleaching and allows simultaneous quantitative determination of fluorescence.
Projection eyepieces (1.67 X to 6.7 X) are used in the microscope head to convey images to a modified image intensifier. Images are captured using a 2/3inch CCD camera, which uses a link to transfer data to a modified frame grabber. Digital image size is 512 x 480 pixels with a 2.5 X projection eyepiece, yielding a spatial resolution of 9.227 μm in the x-axis and 0.191 μm in the y-axis.
The resulting image reconstruction can be manipulated by the microscope operator in all three axes. This provides a stereoscopic image display, image dissections, extraction of areas of interest, and two and three-dimensional tomography.
Source: “Microscope system renders 3-D images”, Vision Systems Design, September, 1997; Biotechnology Research Department, Orange County Water District, Orange, CA
Reference:
1) B. E. Meyers, Redmond, WA
2) Cohu, San Diego, CA
3) Olympus, Melville, NY
3) EG&G, Optoelectronics, Santa Clara, CA
4) Imagraph, Chelmsford, MA
5) Eastman Kodak Co, Rochester, NY
Industrial Products
posted by JD52 @ 7:40 PM,
